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    Agarose extracted from seaweed is used in____________
    Question



    Agarose extracted from seaweed is used in____________

    A.

    Tissue culture

    B.

    Gel electrophoresis

    C.

    Spectroscopy

    D.

    More than one of the above

    E.

    None of the above

    Correct option is B

    Agarose, extracted from seaweed, is widely used in gel electrophoresis for the separation of DNA, RNA, and proteins due to its gel-forming properties.
    Agarose is a natural polysaccharide derived from agar, a substance extracted from red algae (Rhodophyceae).
    Chemical Composition:
    Composed of repeating units of agarobiose ( a disaccharide made up of D-galactose and 3,6-anhydro-L-galactopyranose).
    Physical Properties:
    Forms a gel when dissolved in hot water and cooled.
    High gel strength even at low concentrations, making it suitable for gel electrophoresis.
    Uses in Biotechnology:
    Gel Electrophoresis:
    Widely used in separating DNA, RNA, and proteins based on size.
    DNA fragments migrate through agarose gels under an electric field.
    Molecular Biology:
    For DNA purification and recovery.
    Analysis of PCR products and restriction enzyme digests.
    Chromatography:
    Used in gel filtration and affinity chromatography.
    Concentration and Pore Size:
    The concentration of agarose affects pore size:
    Low concentrations (0.5–1%) are used for separating large DNA fragments.
    High concentrations (2–3%) are used for smaller DNA fragments or oligonucleotides.

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