Correct option is C
Correct Answer:
Option (c) — Differential Interference Contrast (DIC) Microscope
Explanation:
Differential Interference Contrast (DIC) microscopy is ideal for live imaging of unstained, transparent specimens such as C. elegans embryos.
It enhances contrast in transparent samples by exploiting differences in refractive index, allowing detailed visualization of cellular structures without staining or fixation, which are lethal to live embryos.
Information Booster:
-DIC microscopy provides:
High-contrast, pseudo-3D images of transparent cells.
Real-time observation of live developmental events (e.g., cell divisions in embryos).
Minimal photodamage and no requirement for dyes.
It’s widely used in embryology and developmental biology for organisms like C. elegans, Drosophila, and zebrafish.
Additional Knowledge (Incorrect Options Explained):
(a) Confocal microscope: Requires fluorescent labeling and is unsuitable for unstained live embryos.
(b) Fluorescence microscope: Needs staining or fluorescent tags; may cause phototoxicity.
(d) Super-resolution microscope: Provides higher resolution but requires fluorescence and is not practical for live unstained samples.
