Correct option is A
Explanation:
The CRISPR/Cas9 editing system allows for the precise editing of a specific location in the genome by introducing targeted double-strand breaks and then facilitating repairs that lead to specific mutations. This method is highly efficient and precise in creating mutations at a predetermined location in the genome, making it the most precise among the listed strategies.
Information Booster:
CRISPR/Cas9 editing is a revolutionary technique that allows for precise genome editing by guiding the Cas9 enzyme to a specific DNA sequence, where it creates a double-strand break.
The repair of these breaks is carried out by the cell’s repair mechanisms, which can be manipulated to introduce specific mutations, such as insertions or deletions.
CRISPR/Cas9 has transformed genetic research due to its efficiency, precision, and ease of use compared to older methods.
This method allows researchers to modify multiple genes simultaneously (multiplexing) with high specificity.
It is widely used in various organisms, including plants, to study gene function and improve crop traits.
Additional Knowledge:
T-DNA insertion mutagenesis (option 2) involves introducing a foreign DNA segment into the genome, which can cause random insertions and is less precise compared to CRISPR/Cas9.
Transposon mutagenesis (option 3) uses transposons (jumping genes) to insert mutations randomly in the genome. While useful, it is less targeted and precise.
TILLING (option 4) is a method that uses chemical mutagenesis followed by screening for mutations, but it is less precise than CRISPR/Cas9 in directly targeting specific genes.
