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Which one of the following statements regarding PEPCase is INCORRECT?
Question

Which one of the following statements regarding PEPCase is INCORRECT?

A.

​During the day, C4 PEPCase is inactive whereas CAM PEPCase is active.

B.

PEPCase is inactivated by dephosphorylation.

C.

​PEPCase kinase phosphorylates PEPCase.

D.

The synthesis of PEPCase kinase is modulated by circadian rhythm in CAM leaves.

Correct option is A

The question asks for the incorrect statement regarding PEPCase (Phosphoenolpyruvate Carboxylase).

  1. During the day, C4 PEPCase is inactive whereas CAM PEPCase is active.
    • Incorrect. C4 plants and CAM plants regulate PEPCase activity differently. In C4 plants, PEPCase is active during the day to fix CO₂ into Oxaloacetate, whereas in CAM plants, PEPCase is active at night to minimize water loss. Hence, the given statement is incorrect.
  2. PEPCase is inactivated by dephosphorylation.
    • Correct. PEPCase activity is regulated by phosphorylation. The enzyme is activated by phosphorylation and inactivated by dephosphorylation.
  3. PEPCase kinase phosphorylates PEPCase.
    • Correct. PEPCase kinase is responsible for phosphorylating PEPCase, activating it in response to certain environmental conditions.
  4. The synthesis of PEPCase kinase is modulated by circadian rhythm in CAM leaves.
    • Correct. In CAM plants, PEPCase kinase activity follows a circadian rhythm, regulating the phosphorylation state of PEPCase based on the day-night cycle.

Information Booster

  1. PEPCase Role: PEPCase is a crucial enzyme in C4 and CAM metabolism that catalyzes the fixation of CO₂ into oxaloacetate.
  2. C4 Plants: These plants (e.g., maize, sugarcane) use PEPCase to capture CO₂ during the daytime, storing it as malate for later use in the Calvin cycle.
  3. CAM Plants: CAM plants (e.g., cacti, pineapples) fix CO₂ at night using PEPCase to store it as malate, which is then used during the day for photosynthesis.
  4. Regulation of PEPCase: The activity of PEPCase is controlled via phosphorylation. Phosphorylation activates it, while dephosphorylation inactivates it.
  5. Role of PEPCase Kinase: PEPCase kinase is an enzyme that phosphorylates and activates PEPCase, especially in CAM plants.
  6. Circadian Rhythm in CAM Plants: The synthesis of PEPCase kinase in CAM plants is regulated by circadian rhythms, ensuring the enzyme functions optimally according to the day-night cycle

Additional Information

  • Option 2 (Correct Statement: PEPCase is inactivated by dephosphorylation)
    • PEPCase is activated when phosphorylated by PEPCase kinase and inactivated when dephosphorylated.
  • Option 3 (Correct Statement: PEPCase kinase phosphorylates PEPCase)
    • PEPCase kinase is an enzyme that adds a phosphate group to PEPCase, increasing its activity.
  • Option 4 (Correct Statement: PEPCase kinase synthesis is controlled by circadian rhythm in CAM leaves)
    • In CAM plants, the production of PEPCase kinase follows a circadian rhythm, ensuring PEPCase is active at night when CO₂ fixation occurs.

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