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Which is a reversible, fluorescent red, acidic dye used to stain cytoplasm, red blood cells, collagen and muscle fibres for histological examination?
Question

Which is a reversible, fluorescent red, acidic dye used to stain cytoplasm, red blood cells, collagen and muscle fibres for histological examination?

A.

Safranin

B.

Crystal violet

C.

Alizarin

D.

Eosin

Correct option is D


The correct answer is (d) Eosin.
· Eosin is a fluorescent red, acidic dye commonly used in histology to stain cytoplasm, red blood cells, collagen, and muscle fibers.
· It binds to basic (positively charged) components of the cell and tissues, imparting a pink to red coloration.
· Eosin is often used in combination with hematoxylin in the H&E (hematoxylin and eosin) staining protocol, which is a standard technique for examining tissue morphology.
Information Booster:
· Safranin: A basic dye used to stain cell nuclei red in various staining protocols, including Gram staining for bacteria.
· Crystal violet: A basic dye used as a primary stain in Gram staining to differentiate between Gram-positive and Gram-negative bacteria.
· Alizarin: A dye used for staining calcium deposits in tissues, commonly used in bone histology.

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