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    The following statements were made regarding the patterning of anterior-posterior body plan of Drosophila: A. Microinjection of bicoid mRNA in the mid
    Question

    The following statements were made regarding the patterning of anterior-posterior body plan of Drosophila:
    A. Microinjection of bicoid mRNA in the middle of a bicoid-deficient embryo leads to formation of ‘head’ in the middle and telson at the two ends.
    B. Nanos protein inhibits the translation of caudal mRNA at the posterior half of the embryo.
    C. The Bicoid protein activates the zygotic expression of the hunchback gene.
    D. The segment polarity genes are expressed in segments of the embryo.

    Which one of the following options represents all correct statements?

    A.

    A and B only

    B.

    A and C only

    C.

    A, C and D

    D.

    B, C and D

    Correct option is C

    Explanation-

    Statement A - Microinjection of bicoid mRNA in the middle of a bicoid-deficient embryo leads to formation of ‘head’ in the middle and telson at the two ends  - Correct
    Bicoid is a maternal effect gene and acts as a morphogen. It is normally localized at the anterior end, where it specifies head and thorax structures. In a bicoid-deficient embryo, if you inject bicoid mRNA in the center, it creates a new anterior (head) structure in the center, and the posterior ends (telson) form on both sides. This experiment proved that bicoid functions in a concentration-dependent manner to specify positional identity.

    Statement B - Nanos protein inhibits the translation of caudal mRNA at the posterior half of the embryo -  Incorrect
    Nanos is a posterior determinant in Drosophila. However, Nanos inhibits the translation of hunchback mRNA, not caudal. In fact, Bicoid inhibits caudal mRNA translation at the anterior, not Nanos at the posterior.

    Statement C -  The Bicoid protein activates the zygotic expression of the hunchback gene  - Correct
    Bicoid acts as a transcription factor for zygotic hunchback gene expression in the anterior part of the embryo. This is essential for proper head and thorax development.

    Statement D  - The segment polarity genes are expressed in segments of the embryo  - Correct
    Segment polarity genes (e.g., engrailed, wingless) are expressed within each segment and help define the anterior-posterior axis within segments. Their expression maintains segment boundaries and polarity.

    Final Answer: A, C, and D only (Correct option: c)
    B is incorrect because it mixes up the targets of Bicoid and Nanos.









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