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The bacteria that retain the stain in Gram’s method of staining is a:
Question

The bacteria that retain the stain in Gram’s method of staining is a:

A.

Spores of bacteria

B.

Gram positive bacteria

C.

Gram negative bacteria

D.

Broad spectrum bacteria

Correct option is B


Ans. (b)
Sol. In Gram staining, Gram-positive bacteria retain the crystal violet stain, appearing purple under the microscope due to their thick peptidoglycan cell wall, which traps the dye. Gram-negative bacteria do not retain the crystal violet and appear pink after counterstaining with safranin.

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