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Pyruvate generated by glycolysis is converted to acetyl-coenzyme A, which is metabolized by the citric acid cycle generating energy-rich molecules. Fr
Question

Pyruvate generated by glycolysis is converted to acetyl-coenzyme A, which is metabolized by the citric acid cycle generating energy-rich molecules. From the choices given below, select the right combination of these molecules produced from one molecule of acetyl-CoA.

A.

2NADH+2FADH2+1GTP2 NADH + 2 FADH_2 +1 GTP​​

B.

3NADH+1FADH2+1GTP3 NADH+1FADH_2 +1GTP​​

C.

3NADH+1GTP3NADH + 1GTP​​

D.

4NADH+1FADH2+1GTP4NADH +1FADH_2 +1GTP​​

Correct option is B

The correct answer is (b) 3 NADH + 1 FADH₂ + 1 GTP

Explanation:

For each molecule of acetyl-CoA that enters the citric acid cycle (also known as the Krebs cycle), the following molecules are produced:

  1. 3 NADH:
    During the citric acid cycle, 3 NADH molecules are produced through the following reactions:

    • Conversion of isocitrate to α-ketoglutarate (NAD+ is reduced to NADH)

    • Conversion of α-ketoglutarate to succinyl-CoA (NAD+ is reduced to NADH)

    • Conversion of malate to oxaloacetate (NAD+ is reduced to NADH)

  2. 1 FADH₂:
    1 FADH₂ is produced when succinate is converted to fumarate, where FAD is reduced to FADH₂.

  3. 1 GTP (or ATP):
    1 GTP is generated via substrate-level phosphorylation during the conversion of succinyl-CoA to succinate. This GTP can later be converted into ATP.

Thus, from one molecule of acetyl-CoA, the citric acid cycle produces:

  • 3 NADH

  • 1 FADH₂

  • 1 GTP

Information Booster:

  • NADH: NADH is an important electron carrier that transports electrons to the electron transport chain, which is used to generate ATP through oxidative phosphorylation.

  • FADH₂: Like NADH, FADH₂ also carries electrons to the electron transport chain but at a lower energy level than NADH, which results in slightly less ATP generation per molecule of FADH₂.

  • GTP: GTP (guanosine triphosphate) is produced by substrate-level phosphorylation. This GTP can be readily converted to ATP, which provides energy for cellular functions.

Additional Information:

(a) 2 NADH + 2 FADH₂ + 1 GTP
This option is incorrect because the citric acid cycle produces 3 NADH, not 2, from one molecule of acetyl-CoA. The amount of FADH₂ produced is also only 1.

(c) 3 NADH + 1 GTP
This option is incomplete because it omits the 1 FADH₂ that is produced in the citric acid cycle.

(d) 4 NADH + 1 FADH₂ + 1 GTP
This option is incorrect because the citric acid cycle only produces 3 NADH from one acetyl-CoA, not 4.

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