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Plasmid pBR322 has Psl restriction enzyme site within gene that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-g
Question

Plasmid pBR322 has Psl restriction enzyme site within gene that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in a E.coli strain

A.

it will lead to lysis of host cell.

B.

it will be able to produce a novel protein with dual ability

C.

it will not be able to confer ampicillin resistance to the host cell

D.

the transformed cells will have the ability to resist ampicillin as well as produce β—galactoside

Correct option is C

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