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Kerala PSC Scientific Assistant Biochemistry Syllabus 2023, Download PDF

Kerala PSC Scientific Assistant Biochemistry Syllabus: Kerala Public Service Commission has published Kerala PSC Scientific Assistant Biochemistry Syllabus on its official website. If you have applied for the Scientific Assistant in Biochemistry post and would like to know the detailed syllabus, your search ends here. To crack the exam, one needs to have a clear understanding of the syllabus, therefore, read through Kerala PSC Scientific Assistant Biochemistry Syllabus to broaden your perspective. You can also download Scientific Assistant Biochemistry Kerala PSC Syllabus in PDF format.

Kerala PSC Scientific Assistant Biochemistry Syllabus 2023

Kerala PSC Scientific Assistant Biochemistry Syllabus 2023: പരീക്ഷയ്ക്കുള്ള തയ്യാറെടുപ്പുകൾ ആരംഭിക്കാൻ  സമയമായി. പരീക്ഷയിൽ വിജയിക്കുന്നതിന്, സിലബസിനെക്കുറിച്ച് വ്യക്തമായ ധാരണ ഉണ്ടായിരിക്കണം, അതിനാൽ കേരള PSC സയന്റിഫിക് അസിസ്റ്റന്റ് ബയോകെമിസ്റ്ററി സിലബസ് വിശദമായി വായിച്ച് മനസിലാക്കുക. ചുവടെ നൽകിയിരിക്കുന്നു ലിങ്ക് ഉപയോഗിച്ച് നിങ്ങൾക്ക് Kerala PSC Scientific Assistant Biochemistry Syllabus PDF രൂപത്തിൽ ഡൗൺലോഡ് ചെയ്യാവുന്നതാണ്.

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Scientific Assistant Biochemistry Kerala PSC Syllabus 2023

ചുവടെ നൽകിയിരിക്കുന്ന പട്ടികയിൽ Scientific Assistant Biochemistry Kerala PSC Syllabus 2023 സംബന്ധമായ എല്ലാ പ്രധാനപ്പെട്ട വിവരങ്ങളും ലഭിക്കും.

Scientific Assistant Biochemistry Kerala PSC Syllabus 2023
Organization Kerala Public Service Commission
Category Exam Syllabus
Department Medical Education
Name of the Post Scientific Assistant Biochemistry
Category No. 003/2022
Date to Submit Confirmation 22nd April 2023 To 11th May 2023
Mode of Examination ONLINE/ OMR
Medium of Questions English
Total Marks 100
Duration of Examination 1 Hour 30 Minutes
Official Website

Scientific Assistant Biochemistry Exam Pattern

കേരള PSC സയന്റിഫിക് അസിസ്റ്റന്റ് ബയോകെമിസ്റ്ററി പരീക്ഷ പാറ്റേൺ ചുവടെ ചേർക്കുന്നു.

Scientific Assistant Biochemistry Exam Pattern 
Parts Sections Marks
Part I Techniques in Biochemistry 20 Marks
Part II Enzymology 20 Marks
Part III QA, QC, and GLP 15 Marks
Part IV Clinical Biochemistry 20 Marks
Part V Molecular Endocrinology 15 Marks
Part VI Pharmacology 10 Marks

Kerala PSC Scientific Assistant Biochemistry Syllabus PDF Download

Kerala PSC Scientific Assistant Biochemistry Syllabus PDF ഡൗൺലോഡ് ചെയ്യാൻ, താഴെ നൽകിയിരിക്കുന്ന ലിങ്കിൽ ക്ലിക്ക് ചെയ്യുക.

Kerala PSC Scientific Assistant Biochemistry Syllabus PDF Download

Kerala PSC Scientific Assistant Biochemistry Syllabus

സയന്റിഫിക് അസിസ്റ്റന്റ് ബയോകെമിസ്റ്ററി തസ്തികയുടെ വിശദമായ സിലബസ് ചുവടെ ചേർക്കുന്നു.



  • Basic principles, instrumentation, and applications of microscopy. Bright field, Phase-contrast, Fluorescence and Confocal Microscopy, Electron Microscope – Scanning and Transmission Electron Microscopy, and Atomic Force Microscopy. Histopathology – definition, fixation, decalcification, tissue processing, cutting, staining, and analysis.


  • Basic principles, instrumentation, and applications of electrophoresis. Factors affecting electrophoresis. Electrophoretic techniques – Agarose gel, SDS-PAGE, Capillary, 2-D, and Pulsed-field.

PCR and Immunological techniques 

  • DNA amplification by PCR – Conventional, Reverse-Transcriptase, Inverse, Quantitative Real-time, Nested, and Multiplex PCR. Primer designing.


  • Principle, Concept of absorptions, transmission, scattering, phosphorescence, fluorescence, luminescence, diffraction spectra. Principle, instrumentation, working, and application of UV, Visible, and IR spectroscopy, spectro-fluorimetry, flame photometry, atomic absorption spectrometry, and luminometry.

NMR and mass spectrometry 

  •  Principle, instrumentation, working, and application of Nuclear Magnetic Resonance (NMR), Electron Spin Resonance (ESR), Mass spectrometry – GC-MS, HPLC-MS, and LC-MS/MS, Matrix-assisted laser desorption/ionization, Time- of Flight Mass spectroscopy (MALDI-TOF MS), X-ray crystallography


  • Basic principles, Instrumentation, working and applications of partition chromatography (Paper), adsorption chromatography (TLC, HPTLC, different types of columns), affinity chromatography, ion exchange chromatography, gel filtration chromatography, gas-liquid chromatography (GLC), high-pressure liquid chromatography (HPLC).


  • Nomenclature and classification of enzymes. The energy of activation and its significance. Enzyme specificity, measurement, and expression of enzyme activity – Definition of Unit, international unit (IU), katal. Coenzymes- classification – vitamin and nonvitamin coenzymes. Mechanism of enzyme action- Active site, Mechanism of enzyme action – general acid-base catalysis, covalent catalysis, proximity and orientation effects, the role of metal ion in enzyme catalysis, mechanism of serine proteases – chymotrypsin, lysozyme, and ribonuclease.

Enzyme kinetics

  • Order of reaction, progress curve for enzyme-catalyzed reactions. Factors affecting the velocity of enzyme-catalyzed reaction – enzyme concentration, temperature, pH, inhibitors and activators, Michaelis-Menten equation; Km and Vmax values and their significance, Lineweaver-Burk plot, and its physiological significance. Enzyme inhibition – competitive, non-competitive, uncompetitive, and mixed. Allosteric and feedback inhibition with examples, suicide inhibition. Dose-response curves of enzyme inhibition. Regulation of enzyme activity-covalent regulation, allosteric regulation, and Feedback regulation. Multienzyme complex- Mechanism of action and regulation.

Enzyme Technology 

  • Isolation and purification of enzymes and criteria of purity-specific activity. Industrial and therapeutic uses of enzymes. Immobilization of enzymes and their applications. Designer enzymes – Abzymes, Ribozymes. Serum enzymes in health and disease-diagnostic and therapeutic applications.


Quality Assurance- Their functions and advantages, Concept of Quality, Control Quality Standards- Advantages and Disadvantages. Good laboratory practices, WHO guidelines on GLP and GMP, Quality assurances in Good Laboratory Practices.

QA QC in Clinical Laboratory

  • Quality assurance and Quality Control with reference to a clinical laboratory, Factors influencing quality. Quality assurance cycle: Pre analytic, analytic, and post-analytic. QC procedures. Internal quality control and external quality control. Types of errors: Random Error, systematic error. Reasons for laboratory errors. Quality system essentials. Overview of statistics including, calculation of mean, standard deviation, and probability. Monitoring QC data, Levey-Jennings Chart, Westgard rules. Clinical audit of the laboratory, Troubleshooting.


Specimen collection and automation

  • Specimen collection and processing: Blood collection methods, anticoagulants. Cerebrospinal fluid (CSF): Composition and collection, gross examination, cell counts, chemical examination, and bacteriologic examination. Amniotic fluid: Origin, collection, composition, and routine analysis of amniotic fluid. Collection and examination of Synovial fluid, Pleural fluid, Pericardial fluid, and Peritoneal fluid. Collection of urine: Timed urine specimens, and urine preservatives. Basic concepts and definitions of automation in the clinical laboratory. Multifunction Workstations (Automated Specimen Processing), Total Laboratory Automation Systems. Post-assay processing, Signal processing, Data handling and Process control, Instrument clusters, Microtiter plate systems, and Automated pipetting stations.

Clinical Enzymology 

  • Principles of diagnostic enzymology, Factors affecting enzyme levels in the blood. Principle, assay, and clinical significance of transaminases, creatine kinase, lactate, Dehydrogenase, phosphatases, 5’nucleotidase, gamma–glutamyl transferase, amylase, lipase, trypsin, chymotrypsin, choline esterase, glutamate dehydrogenase, and glucose -6-phosphate dehydrogenase. Enzyme pattern in diseases: Myocardial infarction, hepatobiliary diseases.

Organ function tests and related disorders 

  • Liver function test and related disorders: Jaundice, cirrhosis, hepatitis, fatty liver, and gall stones. Renal function test and related disorders: Acute renal failure. glomerular disease, tubular diseases, analysis of urinary calculi. Gastric and pancreatic function tests. Hyper and hypo lipoproteinemias and diagnostic test for lipoprotein disorders. Atherosclerosis, Diabetes, Cancer, Inflammatory arthritis, Obesity – Risk factors, Molecular pathogenesis, Biochemical and clinical features, diagnosis, treatment.


  • Classification of hormones, an overview of circulation, modification, and degradation. Target tissue feedback control. Hormone receptors – general features, structure, and regulation. Mechanism of hormone action. Synthesis, secretion, transport, metabolic fate, biological actions, mechanism of action, functions, and regulation of hormones secreted by the Hypothalamus and Pituitary, Thyroid, Pancreas, adrenal, and Gastrointestinal hormones. Endocrine disorders and their detection methods. Thyroid function tests. Hormones and human health.


  • Introduction to pharmacology, sources of drugs, Classification of drugs based on sources, dosage forms, route of administration, and site of action of drugs. Mechanism of action, the concept of receptors, combined effect of drugs, and factors modifying drug action. Dose-response curve-ED50 and LD50.


  • Absorption and distribution of drugs, the importance of drug–protein interaction. Drug metabolism: chemical pathway of drug metabolism, phase I and phase II reactions, the role of cytochrome P450, non-microsomal reactions of drug metabolism, and drug-metabolizing enzymes. Drug elimination of liver and kidney.

Clinical Toxicology 

  • Definition, and classification of toxicity – occupational, environmental, and pharmaceutical. Types of toxins and their mechanism of action. Factors affecting toxicity-Drug tolerance, intolerance, addiction, allergy, hypersensitivity, antagonism, and synergism.
  • Methods of detection. Drug abuses and their biological effects. Clinical symptoms of toxicity and marker parameters.
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